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61.
阐明植物雄配子体与雌配子体互作的分子机理一直是植物有性生殖研究的前沿和热点。但限于研究难度较大, 很多重要科学问题仍有待回答。关于花粉管如何感知雌配子体信号从而定向生长进入胚囊以投送精细胞就是悬疑多年的问题之一。最近, 中国科学家在解析雄配子体感知雌配子体引导信号的分子机制方面取得了突破性进展。 相似文献
62.
tRNA片段(tRF)是tRNA通过非随机剪切产生的RNA片段, 其产生和功能机制尚不明确; 而在水稻(Oryza sativa)雄配子体发育过程中, 人们对tRNA更是知之甚少。通过高通量测序, 在水稻雄配子体发育过程中发现了长度范围较大的tRFs; 进一步采用logo对tRFs两端的序列进行分析, 发现了4个有序列特征(其中3个未见报道)和1个无序列特征的酶切位点; 通过NCBI Blast预测了tRF靶基因, 发现其大多靶向转座因子。研究结果对揭示tRF产生机制以及水稻雄配子体发育研究有一定的参考价值。 相似文献
63.
Climate changes, particularly the increase of temperature are among the main causes behind the decline of fertility in humans as well as animals. In this study, the effects of heat stress on some reproductive parameters of male cavies and mitigation strategies using guava leaves essential oil (GLEO) were studied. For this purpose, 40 male cavies aged 2.5–3 months and weighing between 348 and 446 g were divided into 4 groups of 10 animals each and subjected to the following temperatures: Ambient temperature (20–25 °C) for the control group, 35 °C for group 1, 45 °C for group 2 and 45 °C+100 µl GLEO/kg body weight, administered by gavage to animals for group 3. Exposure time of heat was 7 h per day for 60 days. Results reveal that the relative weights of testes, epididymis, vas deferens and seminal vesicles were hardly affected by the temperature levels considered (P>0.05). The mass and individual sperm motility was significantly lower (P<0.05) in cavies exposed to the temperature of 35 and 45 °C as compared with those which received GLEO and controls. The percentages of abnormal sperm and altered sperm DNA were higher in animals exposed to temperature of 35 and 45 °C as compared with the controls. The activity of superoxide dismutase significantly increased (P<0.05) in animals exposed to temperature of 45 °C and in those of 45 °C and orally treated with GLEO, compared with cavies exposed to temperature of 45 °C without receiving GLEO. The level of malondialdehyde was significantly increased (P<0.05) in animals exposed to temperature of 35 and 45 °C, whereas the level of nitric oxide was significantly lower (P<0.05) in exposed animals as compared with controls. It was concluded that the exposure of male cavies at 35 and 45 °C for 60 days induce heat stress that causes deterioration of sperm characteristics. These effects that can be mitigated by the administration of guava leaves essential oil. 相似文献
64.
65.
ZHAO Juan CHEN Yan HUI Sheng-Ming TU Wei-Yi CHEN Chen GAO Ju-Fang 《Plant Diversity》2012,34(2):164-170
To identify new genes important for anther development, we screened for male sterile mutants among a population of Arabidopsis ecotype Columbia (Col) mutagenized by T DNA insertion (provided by ARBC). A male sterile mutant line with normal vegetative and flora development but no seed yield was isolated from Salk_118481 line. T DNA insertion site identification showed that there were no T DNA sequences in the genome of the mutants. Genetic analysis indicated that the mutant was controlled by a single recessive nuclear gene named filament no elongation because the filament of the mutant remains very short at the 13-14 stage of anther development. The fne gene was mapped to a region of 97kb between the molecular makers MBD2 and MMG4 on chromosome 5 using map based cloning technique. No genes involved filament elongation were reported in this region, so we believe that FNE gene could be a new gene controlling filament elongation in Arabidopsis. 相似文献
66.
67.
竹叶蕨配子体发育的培养观察 总被引:2,自引:1,他引:1
首次在光学显微镜下观察竹叶蕨孢子及其萌发、丝状体发育、片状体和生长点的形成及分化、原叶体细胞形态、假根及性器的发育等方面所表现出的显微特征。初步讨论竹叶蕨科从鳞始蕨科中分立出来的合理性,以及原叶体边缘细胞的形态、叶绿体对光的敏感性、假根的形态和精子器的形成及分化的系统学意义。 相似文献
68.
用光镜和电镜观察羽叶薰衣草(Lavandula pinnata L.)雄性不育小孢子发育过程的细胞形态学特征.结果表明:羽叶薰衣草花药4枚,每枚花药通常具4个小孢子囊.花药壁发育为双子叶型,从外向内分为表皮、药室内壁、中层和绒毡层4层细胞.减数分裂形成的四分体为四面体及十字交叉型.小孢子的发育过程可分为造孢细胞期、减数分裂时期、小孢子发育早期、小孢子发育晚期.未观察到二胞花粉期和成熟花粉期.羽叶薰衣草花粉败育主要发生在单核花粉时期,细胞内物质解体并逐渐消失变成空壳花粉或花粉皱缩变形成为各种畸形的败育花粉.在此之前小孢子的发育正常.羽叶薰衣草小孢子不育机制体现在绒毡层过早解体、四分体时期以后各细胞中线粒体结构不正常、胼胝质壁与小孢子母细胞脱离、花药壁细胞中淀粉出现时间异常等. 壁发育为双子叶型,从外向内分为表皮、药室内壁、中层和绒毡层4层细胞.减数分裂形成的四分体为四面体及十字交叉型.小孢子的发育过程可分为造孢细胞期、减数分裂时期、小孢子发育早期、小孢子发育晚期.未观察到二胞花粉期和成熟花粉期.羽叶薰衣草花粉败育主要发生在单核花粉时期,细胞内物质解体并逐渐消失变成空壳花粉或花粉皱缩变形成为各种畸形的败育花粉.在此 前小孢子的发育正常.羽叶薰衣草小孢子不育机制体现在绒毡层过早解体、四分体时期以后各细胞中线粒体结构不正常、胼胝质壁与小孢子母细胞脱离、花药壁细胞中淀粉出现时间异常等. 壁发育为双子叶型,从外向内分为表皮、药室内壁、中层和绒毡层4层细胞.减数分裂形成的四分体为四 相似文献
69.
Nicolas Guguen 《Evolution and human behavior》2009,30(5):351-355
Previous research has demonstrated that female behaviors toward men or sexual interest are different across the menstrual cycle. However, women's receptivity to an explicit courtship solicitation still remained in question. In a field experiment, 20-year-old women were approached by 20-year-old male confederates in nightclubs and solicited to dance during the period when slow songs were played. A survey was administered to the women in order to obtain information about the number of days since the onset of previous menses. It was found that women in their fertile phase agreed more favorably to the dance request than women in their luteal phase or in their menstrual phase. 相似文献
70.
Akiko Tamakoshi Koji Suzuki Yingsong Lin Yoshinori Ito Kiyoko Yagyu Shogo Kikuchi Yoshiyuki Watanabe Yutaka Inaba Kazuo Tajima Kei Nakachi for the JACC Study Group 《Mutation Research - Genetic Toxicology and Environmental Mutagenesis》2009,679(1-2):79-83
Cigarette smoking enhances low-grade systemic inflammation in the lung and other organs. Activated immune cells play an important role at early and late stages of inflammation, and in recent years, soluble Fas (sFas), an isoform of death molecule Fas, was found to interfere with the apoptotic pathways of these activated immune cells. The aim of this study was to confirm the association between cigarette smoking and sFas levels in healthy male subjects. We measured serum sFas levels of 4415 male subjects selected as controls for a nested case-control study within the large-scale cohort study conducted in Japan, called the JACC Study. Smoking status at baseline was evaluated by a self-administered questionnaire. Least square means of sFas according to smoking status and numbers of cigarettes smoked per day among smokers were calculated and adjusted for possible confounding factors. Mean sFas levels showed an increasing trend across never smokers, past smokers and current smokers, as 2.21 (95% CI: 2.14–2.27) ng/ml, 2.29 (2.22–2.36) ng/ml, and 2.36 (2.30–2.43) ng/ml, respectively. However, no dose-response relationship was observed between the number of cigarettes smoked per day and sFas levels among smokers. 相似文献